重组工程
同源重组
生物
FLP-FRT重组
遗传学
非等位同源重组
重组
遗传重组
质粒
同源染色体
染色体
Cre-Lox重组
细菌人工染色体
计算生物学
基因
基因组
转基因
转基因小鼠
作者
Donald L. Court,James A. Sawitzke,Lynn C. Thomason
出处
期刊:Annual Review of Genetics
[Annual Reviews]
日期:2002-12-01
卷期号:36 (1): 361-388
被引量:459
标识
DOI:10.1146/annurev.genet.36.061102.093104
摘要
In the past few years, in vivo technologies have emerged that, due to their efficiency and simplicity, may one day replace standard genetic engineering techniques. Constructs can be made on plasmids or directly on the Escherichia coli chromosome from PCR products or synthetic oligonucleotides by homologous recombination. This is possible because bacteriophage-encoded recombination functions efficiently recombine sequences with homologies as short as 35 to 50 base pairs. This technology, termed recombineering, is providing new ways to modify genes and segments of the chromosome. This review describes not only recombineering and its applications, but also summarizes homologous recombination in E. coli and early uses of homologous recombination to modify the bacterial chromosome. Finally, based on the premise that phage-mediated recombination functions act at replication forks, specific molecular models are proposed.
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