生物
驱动蛋白
细胞生物学
高尔基体
枝晶(数学)
信号转导衔接蛋白
转运蛋白
轴突
运动蛋白
小泡
轴浆运输
蛋白质靶向
囊泡转运蛋白
生物物理学
微管
膜蛋白
信号转导
膜
遗传学
内质网
几何学
数学
作者
Andrew C. Montgomery,Christina S. Mendoza,Alex Garbouchian,Geraldine B. Quinones,Marvin Bentley
标识
DOI:10.1091/mbc.e23-10-0401
摘要
Neurons are polarized cells that require accurate membrane trafficking to maintain distinct protein complements at dendritic and axonal membranes. The Kinesin-3 family members KIF13A and KIF13B are thought to mediate dendrite-selective transport, but the mechanism by which they are recruited to polarized vesicles and the differences in the specific trafficking role of each KIF13 have not been defined. We performed live-cell imaging in cultured hippocampal neurons and found that KIF13A is a dedicated dendrite-selective kinesin. KIF13B confers two different transport modes, dendrite- and axon-selective transport. Both KIF13s are maintained at the trans-Golgi network by interactions with the heterotetrameric adaptor protein complex AP-1. Interference with KIF13 binding to AP-1 resulted in disruptions to both dendrite- and axon-selective trafficking. We propose that AP-1 is the molecular link between the sorting of polarized cargoes into vesicles and the recruitment of kinesins that confer polarized transport.
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