Preparation, structural characterization and neuroprotective effects to against H2O2-induced oxidative damage in PC12 cells of polysaccharides from Pleurotus ostreatus

Pleurotus ostreatus is one of the most common edible and medicinal fungi in life, and its polysaccharide has been a hot research topic in recent years. In this paper, a new intracellular polysaccharide component named P. ostreatus polysaccharide (POP-W) was obtained from the mycelium of P. ostreatus, and its structure was analyzed. The results showed that its molecular weight was Mw = 3.034 × 103 kDa, and it did not contain protein and nucleic acid. POP-W was composed of mannose, glucose, galactose and xylose in a molar ratio of 40.34:47.60:7.97:4.09. The backbone of POP-W was α-D-Glcp(1→,→3,4)-α-D-Glcp(1→, →3,4)-α-D-Manp(1→,→3)-α -D-Galp(1→, →4)-α-D-Glcp(1→, →3)-α-D-Glcp(1→, →2)-β-D-Manp(1→, →4) -β-D-Xylp(1 →. SEM and TGA analysis showed the structure of POP-W and good thermal stability. In addition, POP-W showed significant antioxidant activity in vitro. More importantly, POP-W protected PC12 cells induced by H2O2 by inhibiting the contents of lactate dehydrogenase (LDH) and malondialdehyde (MDA) and increasing the levels of superoxide dismutase (SOD) and reduced glutathione (GSH). Western blot detection of Caspase-3, BAX, Bcl-2, PI3K/Akt protein expression. The results showed that POP-W inhibited the expression of caspase-3 and BAX, while promoting the expression of Bcl-2. In addition, POP-W can also promote the phosphorylation of Akt. In conclusion, POP-W pretreatment can protect PC12 cells from H2O2-induced oxidative damage through PI3K/Akt signaling pathway and regulation of apoptosis-related pathway proteins. It provided a theoretical basis for the practical application of the polysaccharide of P. ostreatus in production.