Pro‐Inflammatory Cytokines in Aqueous Humor From ADAMTS10‐Mutant Beagles at an Early Stage of Open‐Angle Glaucoma

ABSTRACT Objective To investigate the level of pro‐inflammatory cytokines in aqueous humor from ADAMTS10 ‐mutant research Beagles at an early stage of open‐angle glaucoma (OAG). Animals Studied Twenty‐four research Beagles were enrolled into this case‐controlled study. Three groups of ADAMTS10 ‐mutant research Beagles were enrolled in this study: (1) Intraocular pressure (IOP) 15–25 mmHg (normotensive IOP), (2) IOP 25–30 mmHg, and (3) IOP 30–36 mmHg. An unaffected control group of Beagles that were either ADAMTS10 ‐wildtype (WT) or heterozygote‐carrier with normotensive IOPs were also enrolled. Procedures Aqueous humor samples were collected and analyzed for 16 pro‐inflammatory cytokines using the Canine Cytokine SpikeMix and target mass spectrometry via multiple reaction monitoring (MRM‐MS). Statistical differences between the four groups' pro‐inflammatory cytokines, as well as correlations between pro‐inflammatory cytokines and IOPs, were analyzed using Kruskal‐Wallis tests and Spearman's rho ( ρ ) correlations, respectively. Results IOP 15–25 mmHg ( n = 8); IOP 25–30 mmHg ( n = 6); IOP 30–36 mmHg ( n = 5); and Control (WT n = 3, carrier n = 2, total n = 5). There were no differences between pro‐inflammatory cytokines across the four groups (all p ‐values > 0.05). IL‐13 had a moderate positive correlation with IOP, but was non‐significant ( ρ = 0.373, p = 0.073); IL‐1β had a moderate negative correlation with IOP but was also non‐significant ( ρ = −0.344, p = 0.100). All other pro‐inflammatory cytokines had only mild correlation with IOPs (| ρ | < 0.229, p > 0.05). Conclusions There were no significant changes in the investigated pro‐inflammatory cytokines with elevated IOP in canine ADAMTS10 ‐OAG.