Abstract Osmotic energy generation has demonstrated significant potential for its use as an alternative renewable energy. In particular, reverse electrodialysis (RED) which exploits a semipermeable membrane to convert osmotic energy into electricity. Recent studies have shown that the use of nanofluidic membranes assembled from two-dimensional (2D) nanosheets in RED systems enhances output power density (P) and energy conversion efficiency (η). MXene membranes, assembled from MXene nanosheets, exhibit highly ordered nanofluidic channels and superior cation-selective permeability, promising candidates for advancing membrane-based RED technologies. In this review, we begin with the preparation and structure of MXene membranes, which dictate the membrane permeance and selectivity and subsequently the P and η for osmotic energy generation. Then we review the application performance of MXene membranes in osmotic energy generation. Finally, we offer a perspective on the research directions for MXene membranes in the advancement of osmotic energy generation technologies.