淫羊藿苷
尼氏体
行为绝望测验
海马体
细胞凋亡
海马结构
药理学
内分泌学
内科学
神经营养因子
细胞色素c
半胱氨酸蛋白酶3
化学
生物
医学
受体
程序性细胞死亡
生物化学
抗抑郁药
病理
染色
替代医学
作者
Xiao Wu,Xiaona Zhang,Lulu Sun,Xiaoming Lu,Cunsi Shen
出处
期刊:Cellular and Molecular Biology
日期:2023-07-31
卷期号:69 (7): 196-204
标识
DOI:10.14715/cmb/2023.69.7.32
摘要
Major depressive disorder (MDD) affects approximately 16% of the global population. Our previous study has demonstrated that icariin (ICA) exhibits anti-depressant activity by increasing the expression of Brain-Derived Neurotrophic Factor (BDNF) in a rat model of chronic unpredictable mild stress (CUMS). In this study, we investigated whether and how ICA can prevent CUMS-induced depression-like behaviors in rats by modulating hippocampus neuronal apoptosis. Forty male rats were randomly divided into control, CUMS, CUMS-fluoxetine (Flx) (10 mg/kg), and CUMS-ICA (20 mg/kg) groups. Behavior tests including sucrose preference test (SPT), open field test (OFT), elevated plus-maze (EPM), and forced swimming tests (FST) were performed. The Nissl staining and TUNNEL assay were used to determine neuronal apoptosis. Subsequently, expression of the glucocorticoid receptor (GR), Bcl-2, cytochrome C, caspase-3 and Bax in the hippocampus was tested by western blot. Our results show that a chronic administration of ICA (20 mg/kg) can prevent CUMS-induced depressant-like behaviors in male model rats. Additionally, ICA significantly inhibited mitochondrial translocation of GR, reduced mitochondrial outer membrane permeabilization (MOMP) to suppress the release of cytochrome C, and then inhibit the activation of caspase-3. In conclusion, our research provides new evidence to understand the anti-depressant activity of ICA, which relates to its inhibition of neuronal apoptosis in the hippocampus through the mitochondrial apoptotic pathway.
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