底漆(化妆品)
适体
DNA
核酸外切酶
核酸
化学
生物
生物传感器
消化(炼金术)
寡核苷酸
生物化学
分子信标
分子生物学
核酸酶
核酸外切酶 III
核酸内切酶
克莱诺碎片
滚动圆复制
生物素化
计算生物学
生物物理学
DNA聚合酶
基因
色谱法
有机化学
大肠杆菌
作者
Roger M. Bialy,Yingfu Li,John D. Brennan
出处
期刊:ChemBioChem
[Wiley]
日期:2021-10-22
卷期号:23 (1)
被引量:3
标识
DOI:10.1002/cbic.202100476
摘要
We report a new method for biosensing based on the target-mediated resistance of DNA aptamers against 5'-exonuclease digestion, allowing them to act as primers for rolling circle amplification (RCA). A target-bound DNA strand containing an aptamer region on the 5'-end and a primer region on the 3'-end is protected from 5'-exonuclease digestion by RecJ exonuclease in a target-dependent manner. As the protected aptamer is at the 5'-end, the exposed primer on the 3'-end can participate in RCA in the presence of a circular template to generate a turn-on sensor. Without target, RecJ digests the primer and prevents RCA from occurring, allowing quantitative fluorescence detection of both thrombin, a protein, and ochratoxin A (OTA), a small molecule, at picomolar concentrations.
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