Use of phosphorylated PAMAM and carboxyled PAMAM to induce dentin biomimetic remineralization and dentinal tubule occlusion

It is crucial to emphasize the biomineralization therapeutic method to repair etched dentin in clinic. Non-collagenous proteins (NCPs) play critical role in the biomineralization of dentine. In this paper, we synthesized the phosphate-terminated polyamidoamine dendrimer (PAMAM-PO3H2) by one-step modification successfully and examined by Fourier-transform infrared spectroscopy (FTIR) and 1H-nuclear Magnetic Resonance (1H-NMR) to characterize the structure of PAMAM-PO3H2. PAMAM-PO3H2 and carboxylterminated dendrimers (PAMAM-COOH) were applied as the dual biomimetic analogs of NCPs. Through the characterization of FT-IR, field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD), the surfaces of human dentin were covered with regenerated crystals and the dentinal tubules were occluded by PAMAM-PO3H2 and PAMAM-COOH. In summary, the combination of PAMAM-PO3H2 and PAMAM-COOH may be another feasible therapeutic method for the treatment of dentin caries and dentin hypersensitivity.