Fabrication of artificial fluorescent protein probe for HSA recognition and relay sensing Ag + by functional microenvironment-sensitive fluorescent dye

Abstract Green fluorescent protein (GFP) becomes one of the most important imaging tools in biology and biomedicine. To simplify usage, expand the application and avoid complicated operations, a kind of artificial fluorescent protein (AFP) is inspired and fabricated by lighting up human serum albumin (HSA) with loading microenvironment-sensitive fluorescent dye DMAR, which could be applied in HSA assay in human urine and serum samples as a physiological indicator of diagnosis in clinical. Besides, the AFP, DMAR/HSA, could act as a nanosensor for Ag+ sensing with good selectivity by a new relay recognition strategy.