废水
线粒体DNA
化学
检出限
环介导等温扩增
人口
DNA提取
色谱法
基因组DNA
污水
核酸
DNA
计算生物学
聚合酶链反应
生物
生物化学
环境科学
基因
环境工程
医学
环境卫生
作者
Zhugen Yang,Gaolian Xu,Julien Reboud,Barbara Kasprzyk-Hordern,Jonathan M. Cooper
标识
DOI:10.1021/acs.analchem.7b02257
摘要
We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic acid biomarkers and shows for the first time the ability to rapidly quantify human-specific mitochondrial DNA (mtDNA) from raw untreated wastewater samples. mtDNA provides a model population biomarker associated with carcinogenesis including breast, renal and gastric cancers. To enable a sample-to-answer, field-based technology, we integrated a filter to remove solid impurities and perform DNA extraction and enrichment into a low cost lateral flow-based test. We demonstrated mtDNA detection over seven consecutive days, achieving a limit of detection of 40 copies of human genomic DNA per reaction volume. The assay can be performed at the site of sample collection, with minimal user intervention, yielding results within 45 min and providing a method to monitor public health from wastewater.
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