内质网
分泌物
体内
分泌蛋白
细胞生物学
分泌途径
化学
体外
高尔基体
生物
生物化学
生物技术
作者
Victor M. Rivera,Xiurong Wang,Scott Wardwell,Nancy L. Courage,Allen Volchuk,Terence P. Keenan,Dennis A. Holt,Michael Gilman,Lelio Orci,Frank Cerasoli,James E. Rothman,Tim Clackson
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2000-02-04
卷期号:287 (5454): 826-830
被引量:315
标识
DOI:10.1126/science.287.5454.826
摘要
A system for direct pharmacologic control of protein secretion was developed to allow rapid and pulsatile delivery of therapeutic proteins. A protein was engineered so that it accumulated as aggregates in the endoplasmic reticulum. Secretion was then stimulated by a synthetic small-molecule drug that induces protein disaggregation. Rapid and transient secretion of growth hormone and insulin was achieved in vitro and in vivo. A regulated pulse of insulin secretion resulted in a transient correction of serum glucose concentrations in a mouse model of hyperglycemia. This approach may make gene therapy a viable method for delivery of polypeptides that require rapid and regulated delivery.
科研通智能强力驱动
Strongly Powered by AbleSci AI