Melatonin activates the OsbZIP79–OsABI5 module that orchestrates nitrogen and ROS homeostasis to alleviate nitrogen-limitation stress in rice

分解代谢 脱落酸 活性氧 氮气 转录因子 发起人 下调和上调 平衡 细胞生物学 化学 新陈代谢 生物 基因 氮缺乏 生物化学 基因表达 有机化学
作者
Meng Jiang,Yue Song,Ruifang Yang,Chenfan Zheng,Yunchao Zheng,Huali Zhang,Shan Li,Yuanyuan Tan,Jianzhong Huang,Qingyao Shu,Ruiqing Li
出处
期刊:Plant communications [Elsevier]
卷期号:4 (6): 100674-100674
标识
DOI:10.1016/j.xplc.2023.100674
摘要

Melatonin (Mel) is previously reported to effectively alleviate nitrogen limitation (N-L) stress and consequently increase nitrogen use efficiency (NUE) in several plants, but the underlying mechanism remains obscure. Here, we revealed that OsbZIP79 (BASIC LEUCINE ZIPPER 79) is transcriptionally activated upon N-L, whose expression is further enhanced by exogenous Mel. By the combined use of omics, genetics, and biological techniques, we uncovered through in-door nitrogen limitation (1/16 normal level) experiments that the OsbZIP79-OsABI5 (ABSCISIC ACID INSENSITIVE 5) route stimulated regulation of reactive oxygen species (ROS) homeostasis, nitrogen uptake and metabolism. Interestingly, OsbZIP79 could activate the transcriptional expression of OsABI5; OsABI5 then binds to promoters of target genes, including genes involved in ROS homeostasis and nitrogen metabolism, and activates their transcription. This route also is indispensable for the upregulation of a few other genes involved in ABA catabolism, nitrogen uptake and assimilation under N-L and Mel, though they are not directly transactivated by OsABI5. Field plot experiments demonstrated that Mel significantly improved rice grown under low nitrogen (L-N, 1/2 normal level), through the same mechanism uncovered in the nitrogen limitation study. Mel application achieved a 28.6% yield increase under L-N, and thus the same level of NUE. Also, the two OsbZIP79 overexpression lines grown in L-N field plots had significantly higher NUE (+13.7% and +21.2%) than its wildtype. Collectively, we discovered an OsbZIP79-OsABI5 route that regulates plant’s response to N-insufficiency (N limitation or low N), which is of importance for increasing NUE in rice production.
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