Microplastic fibers (with a diameter of less than 5 mm) generated by synthetic textiles during the washing machine laundering process are considered to be one of the primary sources of pollution in both water and land environments. This study aimed to investigate the impact of novel combinations of washing parameters on the release of microplastic fibers. A wall-mounted drum washing machine was chosen as the subject of research in order to comprehensively examine the influence of washing conditions on the release of microplastic fibers during the washing process. The results showed that the length of shedding microplastic fibers were between 300 and 400 µm. The diameter of the shedding microplastic fibers was 13 ± 1 µm. High amounts of water and high load can lead to an increase of fiber microplastic release. Abrasion between fabrics influenced the release of microplastic fibers according to the movement of the fabric, which could affect fiber directional migration conditions. The findings on microplastic fibers release will help in the development of better front-loading washers.