DNA Fragment Fusion and Nucleic Acid Detection by Fusion Recombinase-Aided Amplification

(MRSA). Based on fusion RAA, we establish two novel pathogen detection platforms, FREAC (Fusion REcombinase-aided Amplification combined with CRISPR/Cas13a) and FREAL (Fusion REcombinase-aided Amplification combined with Lateral flow assay). Using these two platforms, we can detect clinical MRSA strains within 55 min with high specificity and a limit of detection of 150 copies/μL of genomic DNA, highlighting their potential as user-friendly platforms for nucleic acid detection.