犬淋巴瘤
淋巴瘤
大麻素受体
内大麻素系统
大麻素受体2型
医学
药理学
药效学
BCL10
大麻素
炎症
受体
癌症研究
免疫学
内科学
药代动力学
兴奋剂
作者
Saba Omer,Suhrud Pathak,Rishi M. Nadar,Dylan Bowen,Maninder Sandey,Muralikrishnan Dhanasekaran,Satyanarayana R. Pondugula,Mohammed Mansour,Dawn M. Boothe
出处
期刊:Life Sciences
[Elsevier]
日期:2023-08-01
卷期号:327: 121862-121862
标识
DOI:10.1016/j.lfs.2023.121862
摘要
This study established the in vitro anti-lymphoma pharmacodynamic actions of the endocannabinoids (anandamide-AEA and 2-arachidonoylglycerol-2AG) on canine non-Hodgkin lymphoma (NHL) and human NHL cells.The expression of cannabinoid (CB1 and CB2) receptors in various canine NHL cells {1771, CLBL-1, CLL-1, peripheral blood mononuclear cells (PBMCs)} was studied using Quantitative real-time PCR (RT-qPCR). Anti-lymphoma cell viability assay was performed to assess the effect of endocannabinoids on various canine and human NHL cells (1771, CLBL-1, CLL-1, Ramos cells). The spectrophotometric and fluorometric procedures evaluated oxidative stress, inflammation, apoptosis, and mitochondrial function markers. SAS® and Prism-V La Jolla, CA, USA, were used for statistical analysis.The current study validated the presence of CB1 and CB2 receptors in the canine NHL cells. There was a significantly higher expression of CB1 and CB2 receptors in B-cell lymphoma (BCL) cells (1771, CLBL-1, Ramos) compared to canine T-cell lymphoma (TCL) cells (CL-1). AEA and 2AG dose and time-dependently exhibited significant but differential anti-lymphoma effects on canine and human NHL cells. Anti-lymphoma pharmacodynamic actions of the endocannabinoids in the canine 1771 NHL cells revealed a significant alteration in the markers of oxidative stress, inflammation, and a decrease in mitochondrial function without altering the apoptotic markers.Establishing the anti-lymphoma pharmacodynamic actions of endocannabinoids may provide new therapeutic interventions and expedite cannabinoid research.
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