亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

UCP1-dependent and UCP1-independent metabolic changes induced by acute cold exposure in brown adipose tissue of mice

产热 产热素 褐色脂肪组织 谷氨酰胺 生物化学 化学 糖酵解 生物 内分泌学 内科学 脂肪组织 新陈代谢 氨基酸 医学
作者
Yuko Okamatsu‐Ogura,Masashi Kuroda,Rie Tsutsumi,Ayumi Tsubota,Masayuki Saito,Kazuhiro Kimura,Hiroshi Sakaue
出处
期刊:Metabolism-clinical and Experimental [Elsevier BV]
卷期号:113: 154396-154396 被引量:68
标识
DOI:10.1016/j.metabol.2020.154396
摘要

Abstract

Background

Brown adipose tissue (BAT) is a site of metabolic thermogenesis mediated by mitochondrial uncoupling protein 1 (UCP1) and represents a target for a therapeutic intervention in obesity. Cold exposure activates UCP1-mediated thermogenesis in BAT and causes drastic changes in glucose, lipid, and amino acid metabolism; however, the relationship between these metabolic changes and UCP1-mediated thermogenesis is not fully understood.

Methods

We conducted metabolomic and GeneChip array analyses of BAT after 4-h exposure to cold temperature (10 °C) in wild-type (WT) and UCP1-KO mice.

Results

Cold exposure largely increased metabolites of the glycolysis pathway and lactic acid levels in WT, but not in UCP1-KO, mice, indicating that aerobic glycolysis is enhanced as a consequence of UCP1-mediated thermogenesis. GeneChip array analysis of BAT revealed that there were 2865 genes upregulated by cold exposure in WT mice, and 838 of these were upregulated and 74 were downregulated in UCP1-KO mice. Pathway analysis revealed the enrichment of genes involved in fatty acid (FA) β oxidation and triglyceride (TG) synthesis in both WT and UCP1-KO mice, suggesting that these metabolic pathways were enhanced by cold exposure independently of UCP1-mediated thermogenesis. FA and cholesterol biosynthesis pathways were enhanced only in UCP1-KO mice. Cold exposure also significantly increased the BAT content of proline, tryptophan, and phenylalanine amino acids in both WT and UCP1-KO mice. In WT mice, cold exposure significantly increased glutamine content and enhanced the expression of genes related to glutamine metabolism. Surprisingly, aspartate was almost completely depleted after cold exposure in UCP1-KO mice. Gene expression analysis suggested that aspartate was actively utilized after cold exposure both in WT and UCP1-KO mice, but it was replenished from intracellular N-acetyl-aspartate in WT mice.

Conclusions

These results revealed that cold exposure induces UCP1-mediated thermogenesis-dependent glucose utilization and UCP1-independent active lipid metabolism in BAT. In addition, cold exposure largely affects amino acid metabolism in BAT, especially UCP1-dependently enhances glutamine utilization. These results contribute a comprehensive understanding of UCP1-mediated thermogenesis-dependent and thermogenesis-independent metabolism in BAT.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
4秒前
uo发布了新的文献求助10
5秒前
金蚕完成签到,获得积分20
18秒前
slby完成签到,获得积分10
24秒前
bixiao应助科研通管家采纳,获得20
25秒前
bixiao应助科研通管家采纳,获得20
25秒前
量子星尘发布了新的文献求助10
26秒前
51秒前
寰2023发布了新的文献求助10
55秒前
量子星尘发布了新的文献求助10
1分钟前
寰2023完成签到,获得积分10
1分钟前
uo完成签到,获得积分10
1分钟前
uo发布了新的文献求助10
1分钟前
1分钟前
量子星尘发布了新的文献求助10
1分钟前
1分钟前
qqJing完成签到,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
量子星尘发布了新的文献求助10
2分钟前
拼尽全力发布了新的文献求助10
2分钟前
2分钟前
华仔应助科研通管家采纳,获得10
2分钟前
2分钟前
量子星尘发布了新的文献求助10
2分钟前
3分钟前
临河盗龙发布了新的文献求助20
3分钟前
111发布了新的文献求助10
3分钟前
李健应助111采纳,获得10
3分钟前
量子星尘发布了新的文献求助30
3分钟前
冷静的访天完成签到 ,获得积分10
3分钟前
3分钟前
量子星尘发布了新的文献求助10
4分钟前
4分钟前
banbieshenlu发布了新的文献求助10
4分钟前
可夫司机完成签到 ,获得积分10
4分钟前
量子星尘发布了新的文献求助10
4分钟前
JamesPei应助banbieshenlu采纳,获得30
4分钟前
4分钟前
TTTTTT发布了新的文献求助10
4分钟前
高分求助中
Africanfuturism: African Imaginings of Other Times, Spaces, and Worlds 3000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 2000
Electron microscopy study of magnesium hydride (MgH2) for Hydrogen Storage 1000
Structural Equation Modeling of Multiple Rater Data 700
 Introduction to Comparative Public Administration Administrative Systems and Reforms in Europe, Third Edition 3rd edition 590
全球膝关节骨性关节炎市场研究报告 555
Exhibiting Chinese Art in Asia: Histories, Politics and Practices 540
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3892401
求助须知:如何正确求助?哪些是违规求助? 3435160
关于积分的说明 10791469
捐赠科研通 3160116
什么是DOI,文献DOI怎么找? 1745365
邀请新用户注册赠送积分活动 842857
科研通“疑难数据库(出版商)”最低求助积分说明 786887