飞秒
显微镜
光学
荧光
双光子激发显微术
材料科学
荧光寿命成像显微镜
荧光显微镜
吲哚青绿
光子计数
临床前影像学
激发态
光子
显微镜
激光器
核磁共振
体内
物理
原子物理学
生物
生物技术
作者
Hui Cheng,Sarah Y. Tong,Xiangquan Deng,Hongji Liu,Yu Du,Chen He,Ping Qiu,Ke Wang
出处
期刊:Optics Letters
[The Optical Society]
日期:2019-08-30
卷期号:44 (17): 4432-4432
被引量:31
摘要
Here we demonstrate deep-brain 2-photon fluorescence microscopy in mouse in vivo, excited at the 1700 nm window. Through signal versus power measurement, we show that indocyanine green (ICG) is a promising 2-photon fluorescent dye excitable at the 1700 nm window. In order to excite ICG circulating in the vasculature in the deep brain, we employ a circular-polarization soliton self-frequency shift technique to generate energetic femtosecond pulses at 1617 nm. Combining the labeling and laser technologies, we achieve a record 2-photon fluorescence brain vasculature imaging depth of 2000 μm in vivo. Both the effective attenuation length measurement and signal-to-background ratio measurement indicate that we have reached the theoretical depth limit in 2-photon fluorescence microscopy.
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