适体
膜
化学
细胞
分离(微生物学)
结扎
纳米技术
材料科学
生物
生物化学
分子生物学
生物信息学
作者
Qianqian Gao,Yumeng Zhao,Kangli Xu,Chao Zhang,Qian Ma,Liqing Qi,Dandan Chao,Tingting Zheng,Linlin Yang,Yanyan Miao,Da Han
标识
DOI:10.1002/anie.202011198
摘要
Abstract A single‐step method for isolation of specific cells based on multiple surface markers will have unique advantages because of its scalability, efficacy, and mildness. Herein, we developed multi‐aptamer‐mediated proximity ligation method on live cell membranes that leverages a multi‐receptor co‐recognition design for enhanced specificity, as well as a robust in situ signal amplification design for improved sensitivity of cell isolation. We demonstrated the promising efficacy of our method on differentiating tumor cell subtypes in both cell mixtures and clinical samples. Owing to its simple and fast operation with excellent cell isolation sensitivity and accuracy, this approach will have broad applications in biological science, biomedical engineering, and personalized medicine.
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