HEK 293细胞
胚胎干细胞
重组DNA
转染
异源的
生物
生物制药
细胞生物学
细胞培养
计算生物学
生物化学
生物技术
基因
遗传学
作者
Jiayin Hu,Jizhong Han,Haoran Li,Xian Zhang,Lan Lan Liu,Fei Chen,Bin Zeng
出处
期刊:Cells Tissues Organs
[S. Karger AG]
日期:2018-01-01
卷期号:205 (1): 1-8
被引量:52
摘要
Mammalian cells, e.g., CHO, BHK, HEK293, HT-1080, and NS0 cells, represent important manufacturing platforms in bioengineering. They are widely used for the production of recombinant therapeutic proteins, vaccines, anticancer agents, and other clinically relevant drugs. HEK293 (human embryonic kidney 293) cells and their derived cell lines provide an attractive heterologous system for the development of recombinant proteins or adenovirus productions, not least due to their human-like posttranslational modification of protein molecules to provide the desired biological activity. Secondly, they also exhibit high transfection efficiency yielding high-quality recombinant proteins. They are easy to maintain and express with high fidelity membrane proteins, such as ion channels and transporters, and thus are attractive for structural biology and electrophysiology studies. In this article, we review the literature on HEK293 cells regarding their origins but also stress their advancements into the different cell lines engineered and discuss some significant aspects which make them versatile systems for biopharmaceutical manufacturing, drug screening, structural biology research, and electrophysiology applications.
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