Rapid Identification of Anthocyanin in Ficus Aurata Fruits by Liquid Chromatography-Mass Spectrometry Approach

The aim of this study was to identify the chemical structure of anthocyanins in the fruits of Ficus Aurata. The anthocyanin was detected and characterized using the liquid chromatography system with UV-VIS detection tandem triple quadrupole mass spectrometer. After UV-VIS detection, the characterization of anthocyanin was subjected to a triple quadrupole system of mass spectrometer. The precursor ions of anthocyanidins (cyanidin, delphinidin, malvidin, pelargonidin, petunidin, and peonidin) were scanned to identify the distinctive particular anthocyanin. Then, the detected anthocyanins was further confirmed and their isomers such as glycosides and galactosides forms were distinguished by the fragmentation pattern on product-ion analysis scan after comparison with mass spectroscopy online databases. For the first time, it had characterized that in the fruits of Ficus Aurata contains at least seven kinds of anthocyanins with all possible combinations of three anthocyanidins.