生物
家蚕
多面体
病毒复制
重组DNA
分子生物学
病毒学
转染
基因
细胞质
信号转导衔接蛋白
细胞生物学
病毒
遗传学
夜蛾
作者
Zi Liang,Renyu Xue,Xing Zhang,Man Cao,Sufei Sun,Yunshan Zhang,Min Zhu,Ziyao Zhang,Kun Dai,Jun Pan,Guangwen Cao,Chonglong Wang,Xiaolong Hu,Chengliang Gong
标识
DOI:10.1016/j.ijbiomac.2022.03.213
摘要
β-Arrestin 2 is known to be a widely distributed adaptor protein in mammals but its function has never been reported in Lepidoptera insects. Herein, the β-Arrestin 2 (BmArrestin 2) gene from silkworm was cloned and characterized. The spatiotemporal expression level of BmArrestin 2 was highest in the gonads at the 3rd day of 5th instar, whereas the highest and lowest abundance of BmArrestin 2 were identified in the tracheal and testis, respectively. BmArrestin 2 is mainly distributed in the cytoplasm. Furthermore, in BmN cells,overexpression of BmArrestin 2 promoted Bombyx mori nucleopolyhedrovirus (BmNPV) and B. mori cytoplasmic polyhedrosis virus (BmCPV) replication as the increment of the concentration of plasmid transfection, whereas silencing the gene with specific siRNA inhibited viral replication. Replication of BmNPV and BmCPV also was weakened using BmArrestin 2 antiserum as the increment of the concentration. Immunofluorescent staining revealed the invasion of recombinant BmNPV or BmCPV was decreased after blocking endogenous BmArrestin 2. On the other hand, BmArrestin 2 co-localizes with recombinant BmNPV and BmCPV virions in BmN cells. These results suggest that BmArrestin 2 may represent a novel target for antiviral strategies, as it is an adaptor protein that plays a key role in virus replication.
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