再生(生物学)
器官发生
生物
胚胎发生
植物组织培养
组织培养
外植体培养
细胞生物学
微繁殖
植物
胚胎
胚胎发生
遗传学
体外
基因
作者
Yun Long,Yun Yang,Guangtang Pan,Yaou Shen
标识
DOI:10.3389/fpls.2022.926752
摘要
Plant regeneration occurs when plants repair or replace damaged structures based on the totipotency and pluripotency of their cells. Tissue culture is one of the most widely used regenerative technologies. Recently, a series of breakthroughs were made in the study of plant regeneration. This review summarizes two regenerative pathways in tissue culture: somatic embryogenesis and de novo organogenesis. Furthermore, we review the environmental factors influencing plant regeneration from explant sources, basal culture medium, plant growth regulators, and light/dark treatment. Additionally, we analyse the molecular mechanisms underlying two pathways. This knowledge will promote an understanding of the fundamental principles of plant regeneration from precursor cells and lay a solid foundation for applying plant micropropagation and genetic modification.
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