The Expression of LDH and MDH in Cornea Cautery with Alkali and Its Significances of Energy Metabolism in Rat

Objective:To study the expresson of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cornea after cautery with alkali and explore its effect on energy metabolism in rats.Method:The levels of LDH and MDH were detected by electrophoresis methods.The changes of energy metabolism were analyzed.Result:The percentage content of LDH and MDH changed obviously by scanning.In Early stage of cautery,thelevels of LDH 1,LDH 2,LDH 3 and LDH 4 decreased,that of LDH 5 increased,The level of LDH 5 recovered from 7 to 10 days later.Compared with controls,the level of MDH 3 showed significant difference.Conclusion:Influenced by inflammatory immunoreaction,the content of enzyme involved in energy metabolism in cornea changes,then the condition of energy metabolism changes after corneal alkali cautery.The anoxyglycolysis which increased with MDH 1,MDH 2 and LDH 5 providesenergy for cornea.