The outer coat protein gene was used as target gene for phylogenetic analysis of viruses in Reoviridae reported in the 8th ICTV report.Based on the amino acid sequences,phylogenetic analysis from the neibor-joining(NJ) method and the maximum likehood(ML) method were performed respectively.The results showed that the topology of phylogenetic tree of two methods was consistent with each other.Though there were differences between the two methods in partial branches of phylogenetic tree,the topology could reveal the phylogenetic relationship among genera and tribes,which supported the taxonomic position of the family Reoviridae.In addition,the similarities and differences between the two methods were compared and the reason for differences was discussed.