Detecting a novel CXC chemokine macrophage inflammatory protein-2γ mRNA by real-time fluorescent quantitative reverse transcription polymerase chain reaction (TaqMan)

Objectives To develop and evaluate a real-time fluorescent quantitative RT-PCR method for the detection of a novel CXC chemokine macrophage inflammatory protein-2γ(MIP-2γ)mRNA expression based on TaqMan technique.Method The expression of MIP-2γ mRNA was detected by TaqMan real time fluorscet quantitative RT-PCR. Results The dynamic range of the assay varied from 102 to 107 copies. The intra- and inter-assay coefficient variation (CV) were less than 122%. MIP-2γ mRNA is expressed in heart, liver and kidney of normal Balb/c mice, with the greatest expression in heart.Conclusion The detection of MIP-2γ mRNA expression by real time fluorescent quantitative RT-PCR based on TaqMan technique is more accurate,specific, sensitive and time-saved, which is suitable for use in clinical laboratory.