Role of chemokine IP-10 and its receptor CXCR3 in acute rejection after liver transplantation in rats

Objective To explore the dynamic changes of the expression of chemokine IP-10 and its receptor CXCR3 before and after liver transplantation in rats and to analyze the relationship between the role and the acute rejection(AR).Methods The model of orthotopic liver transplantation(OLT) of rats was established by two-cuff technique.Rats were randomed into 4 groups: Operation injury,OLT without AR,OLT with AR and OLT treated by FK506.The dynamic expression of IP-10 and CXCR3 was measured 1 day prior to OLT and 1,3,5,7 days and after OLT.Results ①The expression of chemokine IP-10 and its receptor CXCR3 in peripheral blood increased significantly in each group after operation.②On the fifth day after OLT,the concentration of chemokine IP-10 and the expression of CXCR3 in AR group were significantly higher than those in the other groups(P0.01);③The IP-10 and CXCR3 in peripheral blood was significantly correlated with RAI on the 7th day after OLT,the correlation coefficients were 0.89 and 0.92(P0.05).Conclusion The dynamic expression of chemokine IP-10 and its receptor CXCR3 in the peripheral blood is closely related to acute rejection and can be used as a diagnostic indicator.