HPLC,paper chromatography and Berthelot spectrophotometry were recommended as three methods to assay the content of γ-Aminobutyric acid(GABA) existing in food materials in the present article.It was seen that HPLC,as a widely used method,was sensitive and accurate in GABA analysis.Paper chromatography and Berthelot spectrophotometry,as common methods to assay GABA,were easy for GA-BA analysis from a lot of samples in practice.Moreover,paper chromatography might be used to detect the GABA contained in food materials like fermentation broth or less pigment.Berthelot spectrophotometry might be employed to detect GABA existing in food materials with sim-ple chemical composition.