Expression and Construction of Bovine Enterokinase Light Chain in Recombinant Escherichia Coli

Enterokinase is a serine protease that recognizes Asp-Asp-Asp-Asp-Lys sequence and cleaves the C-terminal peptide bond of the lysine residue.Design eighteen primers according to the sequence of NCBI,slight chain of bovine enterokinase gene was synthesized by SOE-PCR.Construct the expression vector(pET32a-EK).Then the plasmid was transformed into E.coli BL21(DE3)for expression.IPTG was added to express the fusion protein,use lon exchange column to purificy the enterokinase.The purified enterokinase was obtained which would be able to lay foudation for ite further application of products on a large scale.