Influence of cryopreservation on the dehydrogenase activity of Castanopsis sclerophylla seeds and excised embryos

Triphenylformazane(TTCH) contents of seeds,excised embryos pretreated with or without cryoprotectants,were(cryopreserved) and then measured for the cryopreservation effects.The results showed that TTCH contents of excised embryos (cryopreserved) with cryoprotectants pretreatment were higher than without the pretreatment and seeds after cryopreservation.12%-15% moisture content(MC),especially 15% MC of seeds,as well as mild freezing and rapid thawing,were suitable treatment to be cryopreserved,and got a higher TTCH contents,24.93 and 27.43 μg·mL~-1,respectively.10%-15% MC,(especially) 12% of excised embryos was suitable,larger than seeds range too.Moreover,excised embryos were pretreated with(cryoprotectants) and cryopreserved,TTCH content of excised embryos with 15%-20% MC,36.98 and 31.58 μg·mL~-1,were higher than the other MC levels.It was significantly protective effects of cryoprotectants during the period of cryopreservation.The treatment of 10% DMSO got a terrific effect on TTCH content.