Genetic analysis and mapping of gl-2 gene in cucumber (Cucumis sativus L.)

Genetic analysis and gene mapping were carried out on gl-2 gene in cucumber(Cucumis sativus L.)using 9110G(twith trichomes)and NCG-042(glabrous mutant)as experimental materials.The genetic analysis showed having trichomes or not is determined by a single nuclear gene,and the trait of having trichomes(Gl-2)is dominant to the glabrous(gl-2)in cucumber.Bulked segregant analysis(BSA) and simple sequence repeat(SSR)techonology were employed to mapping gl-2 gene of cucmber in F2 population.gl-2 gene was mapped to a linkage group with 11 SSR makers,corresponding to chromosome 2 of cucumber.The flanking markers SSR10522 and SSR132751 were linked to the gl-2 gene with genetic distances of 0.6 and 3.8 cM,respectively.The veracity of SSR10522 and SSR132751 was tested using BC1P2 population,and the accuracy rate for the two markers was 94.4% and 91.6%.