NASSAM for simultaneous estimation of berberine and curcumin in polyherbal formulation

T newly developed Net Analyte Signal Standard Addition Method (NASSAM) for the simultaneous estimation of berberine and curcumine was simple, specific, accurate, precise, rapid, sensitive and economical which indicate its adequacy for routine pharmaceutical analysis. NASSAM is used for the spectrophotometric determination of analyte in the multicomponent system. Spectrophotometric measurements were made on Perkin Elmer Lambda 35 UV/visible spectrophotometer coupled with computer loaded with UV Win Lab PC software version 6.0.3. All spectra were scanned at 210-520 nm and saved in CSV format and then data were statistically analysed by unscrambler® 10.2. The LOD and LOQ were found to be 0.063 μg/mL and 0.207 μg/mL for berberine and 0.019 μg/mL and 0.065 μg/mL for curcumin, respectively. The percentage purity of the market formulation Talekt for berberine and curcumin were found to be 94.13% w/w and 96.98% w/w, respectively. This method was simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing for the marketed polyherbal formulations. Nitin Sharma et al., J Anal Bioanal Tech 2013, 4:5 http://dx.doi.org/10.4172/2155-9872.S1.015