Production of Trichoderma reesei Cellulase System with High Hydrolytic Potential by Solid-State Fermentation

For efficient and complete hydrolysis of cellulose, cellulase systems must contain endoglucanase, exoglucanase, and β-glucosidase in the right proportions. The cellulase systems produced under typical laboratory culture conditions are usually deficient in β-glucosidase. We show here that with solid-state fermentation, as fungal cultures are adapted to grow in nature, Trichoderma reesei QMY-1 produces high levels of cellulase enzymes, often having higher activities and more optimal β-glucosidase levels than with other methods. Consequently, this cellulase system has high hydrolytic potential. The presence of cotton hydrolyzing activity in this cellulase system could also be related to its high hydrolytic potential.