乙型肝炎表面抗原
RNA干扰
小干扰RNA
转基因
生物
乙型肝炎病毒
小RNA
内生
病毒学
基因沉默
RNA沉默
核糖核酸
基因
细胞生物学
病毒
生物化学
作者
Shuo Zhang,Xiaolin Sang,De‐Xing Hou,Jinmei Chen,Hang Gu,Yujing Zhang,Jing Li,Darong Yang,Haizhen Zhu,Xiao Yang,Fangyu Wang,Chenyu Zhang,Xi Chen,Ke Zen,Chenyu Zhang,Zhi Hong
出处
期刊:Biomaterials
[Elsevier]
日期:2019-07-01
卷期号:210: 83-93
被引量:23
标识
DOI:10.1016/j.biomaterials.2019.04.033
摘要
Chronic infection with hepatitis B virus (HBV) is a major public health problem. Recently, RNA interfering-based strategy has shown great potential to eradicate HBV infection. In current study, we report the experimental observation of plant-derived artificial microRNAs (amiRNAs) acting as therapeutics in HBsAg−/+ transgenic mice. Two pieces of small silencing RNA sequences, siR471 and siR519, against HBV surface antigen gene (HBsAg) were designed and expressed in lettuce using plant endogenous microRNA biogenesis machinery. Administration of amiRNAs-containing lettuce decoction specifically inhibited the HBsAg gene expression. In long term treatments, the liver injury in HBsAg−/+ transgenic mice were alleviated and no toxicological effects were observed. Compared with synthetic siRNA, feeding amiRNAs at a lower level achieved a similar inhibitory effect on HBsAg expression in mice. These results strongly suggest that employing plant endogenous miRNA biogenesis machinery to generate medicinal siRNAs is a novel way to solve the problems of siRNA stability and reduce the potential side effects of RNAi therapy.
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