下调和上调
癌症研究
PI3K/AKT/mTOR通路
生物
U87型
微阵列分析技术
细胞培养
小桶
体外
脑瘤
胶质瘤
信号转导
基因
转录组
计算生物学
细胞生物学
基因表达
病理
遗传学
医学
作者
Liang Ma,Bin Zhang,Changchun Zhou,Yuting Li,Binjie Li,Mengfei Yu,Yichen Luo,Lei Gao,Duo Zhang,Qingwu Xue,Qingchong Qiu,Biaoyang Lin,Jun Zou,Huayong Yang
标识
DOI:10.1016/j.colsurfb.2018.09.034
摘要
GBM, the most common and aggressive malignant primary brain tumors which needs new research approach to reveal the underline molecular mechanism of tumor progression. The 3D in vitro tumor model can be a simple and effective way to study tumor characteristics with ability to replicate of the tumor milieu. In the current study, we adopted the DNA microarray to analyze the gene expression of GBM tumor cells cultured under 2D cell culture flasks and 3D PLA porous scaffolds for 4,7 and 14 days. For 14 day old cultures, 8117 and 3060 genes expression were upregulated and downregulated respectively. Further KEGG pathway analysis revealed, the upregulated genes were mainly enriched/involved in PPAR and PI3K-Akt signaling pathways whereas the downregulated genes were mainly contributed in metabolism, ECM related and TGF-beta pathways. Thus, our approach of establishing 3D in vitro tumor model provides realistic results and proves itself a powerful tool for understanding the inner nature of GBM and can be considered as potential platform for drug screening.
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