纳米探针
生物物理学
线粒体
细胞质
荧光
膜电位
化学
DNA
荧光寿命成像显微镜
纳米技术
生物化学
生物
材料科学
纳米颗粒
物理
量子力学
作者
Zhichao Liu,Hao Pei,Limin Zhang,Yang Tian
出处
期刊:ACS Nano
[American Chemical Society]
日期:2018-11-12
卷期号:12 (12): 12357-12368
被引量:118
标识
DOI:10.1021/acsnano.8b06322
摘要
Herein, a single highly selective DNA nanoprobe was designed and created for the real-time imaging and simultaneous quantification of two kinds of biological species using Ca2+ and pH; the molecules were selected as models because of their close relationship with cellular functions and diseases. A Ca2+ fluorescent probe was synthesized and assembled onto a DNA nanostructure together with pH-responsive, inner-reference, and mitochondria-targeted molecules. This nanoprobe with high spatial resolution, together with long-term fluorescent and structural stability, powerfully tracked pH and Ca2+ dynamics at the same localization in mitochondria in response to O2•--induced oxidative stress and aggregated amyloid β (Aβ) stimulation with a temporal resolution of milliseconds. Using this tool, we discovered that O2•- and Aβ triggered transitory cytoplasmic acidosis and then activated acid-sensing ion channel 1a (ASIC1a) in the mitochondrial membrane, leading to mitochondrial Ca2+ overload and pH abnormalities, which contribute to neuron death. Moreover, psalmotoxin 1 effectively protected against O2•-- and Aβ-induced neuron injury.
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