Identification of immune-related genes in gills of Chinese mitten crabs (Eriocheir sinensis) during adaptation to air exposure stress

The Chinese mitten crab, Eriocheir sinensis, is the most important crab in China. Air exposure is regarded as one of the crucial restriction factors in the crab cultivation and transportation process. Numerous studies have shown that air exposure stress can cause many negative effects on aquatic farming animals. However, the molecular mechanisms of drying on Chinese mitten crabs are still poorly studied. In this study, gill reference transcriptome was assembled and differentially expressed gene (DGE) analysis was conducted between air exposure 16 h and normal dissolved oxygen of Chinese mitten crab. A total of 76075 transcripts were generated and 50800 unigenes with a mean length of 1090 bp and N50 length of 1584 bp were observed. Transcriptomic comparison revealed 352 DEGs between air exposure 16 h group and control group, including 122 up-regulated genes and 230 down-regulated genes. Gene ontology (GO) analysis revealed that these DEGs involved in 16 biological process subcategories, 8 cellular component subcategories and 6 molecular function subcategories. Further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis annotated 352 DEGs to 85 pathways, and some pathways were regarded as related with immune system and diseases, such as phagosome, systemic lupus erythematosus, and alcoholism. Eight genes involved in multiple KEGG signaling pathways were validated by qRT-PCR. This study demonstrates the first gill transcriptomic analysis challenged with air exposure stress in Chinese mitten crab and provides valuable gene resources for understanding the crab gill immunity, which can provides insight into the immune response of crab against air exposure stress.