Enzyme-free and rapid visual quantitative detection for pesticide residues utilizing portable smartphone integrated paper sensor

Serious toxicity for organisms from pesticide glyphosate (Gly) residues to the ecosystem and human health has become a consensus. Rapid and selective detection of glyphosate, especially using a simple and portable instrument, is highly desired. In this work, we develop a novel enzyme-free rapid and visual ratiometric fluorescence sensor for selectively quantitative detecting glyphosate by integrating the designed blue carbon nanodots (CDs) and gold nanoclusters (Au NCs). The fluorescence of CDs can be quickly quenched via aggregation-caused quenching (ACQ) within 2 s after introducing glyphosate, resulting from the formation of CDs-Gly-CDs complex aggregation. While the Au NCs serve as the reference signal without any change, therefore leading to obvious and instant ratiometric fluorescence variation from blue to pink to orange. The broad linear range was obtained from 0 to 180 nM with a satisfactory detection limit of 4.19 nM. Furthermore, this approach was successfully applied to detect glyphosate in real samples and a portable smartphone platform integrated paper sensor was developed for in-site visual quantitative glyphosate detection, offering a promising strategy for the construction of enzyme-free trace hazard detection system.