Plant Growth-Promoting Activity of Herbaspirillum aquaticum ZXN111 on the Zijuan Tea Plant (Camellia sinensis var. assamica)

Herbaspirillum aquaticum ZXN111 which was isolated from the tea plant Zijuan can produce indole-3-acetic acid (IAA) and contain abiotic-stress tolerance gene 1-aminocyclopropane-1-carboxylate deaminase (accd). In this study, ZXN111 PGP activity and the molecular mechanism were investigated. The result showed that ACCD activity of wild-type ZXN111 was 0.4505 mM α-KB/mg·Pro·h, but mutants Δacc and Δacc-tyrb did not showed ACCD activity. IAA production by ZXN111 within 48 hrs was 20.4 μg/mL, while mutants of Δtyrb and Δacc-tyrb were lower than 3.6 μg/mL, indicating that indole-3-pyruvic acid is the primary IAA synthesis pathway. Potting tests found that ZXN111 displayed significant PGP activity to the tea plant Zijuan, but Δtyrb and Δacc-tyrb did not show PGP activity, indicating that IAA is critical to PGP activity. In a salt-stress test, ZXN111 did not enhance the tea plant NaCl tolerance by gene accd. The results of this study indicated that strain ZXN111 has potential for biofertilizer development on tea plantation.