谷氨酰胺转移酶
酿酒酵母
生物化学
ATP合酶
色氨酸合酶
谷氨酰胺
化学
生物合成
吲哚试验
生物
氨基酸
酶
基因
色氨酸
作者
Franziska Prantl,Alexander W.M. Strasser,Markus Aebi,Rolf Furter,Peter Niederberger,Kasper Kirschner,R Huetter
出处
期刊:European journal of biochemistry
[Wiley]
日期:1985-01-01
卷期号:146 (1): 95-100
被引量:27
标识
DOI:10.1111/j.1432-1033.1985.tb08624.x
摘要
The indole‐3‐glycerolphosphate synthase/anthranilate synthase complex from Saccharomyces cerevisiae was purified to apparent homogeneity. The native complex with M r ∼ 130000 consists of two different subunits, the TRP2 gene product with M r = 64000 and the TRP3 gene product with M r = 58000. The larger polypeptide was identified as anthranilate synthase and is active in vitro with ammonia as cosubstrate without need of complex formation. The smaller polypeptide carries both glutamine amidotransferase activity and indole‐3‐glycerolphosphate synthase activity. Various steady‐state kinetic parameters as well as the amino acid composition of the two polypeptides were determined.
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