淋巴系统
异硫氰酸荧光素
解剖
病理
淋巴
淋巴结
医学
荧光
量子力学
物理
作者
Rakesh K. Jain,Lance L. Munn,Dai Fukumura
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2012-11-01
卷期号:2012 (11): pdb.prot072116-pdb.prot072116
被引量:3
标识
DOI:10.1101/pdb.prot072116
摘要
The lymphatic system is an important route for metastasis of cancer cells. Lymphatic microvessels have been studied by adapting lymphangiography to the mouse tail. In this technique, a high-molecular-weight fluorescently labeled tracer is injected into the interstitial compartment of the tail tissue. The tracer migrates to the local lymphatic vessels and fills the network, allowing visualization. By implanting a tumor in the tail, the structure and function of the lymphatics at the tumor periphery can be monitored. This protocol describes a method for injecting tumor cells into a mouse tail and for microlymphangiography.
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