中性粒细胞胞外陷阱
细胞外
生物
细胞质
刺激
细胞生物学
呼吸爆发
DNA
分子生物学
生物化学
免疫学
内分泌学
炎症
作者
Phongsakorn Chuammitri,Jelena Ostojić,Claire B. Andreasen,Sarah Redmond,Susan J. Lamont,Jelena Palić
标识
DOI:10.1016/j.vetimm.2008.12.013
摘要
Recent findings in mammals and fish have revealed that neutrophil nuclear material associated with cytoplasmic granular content is released in the form of neutrophil extracellular traps (NETs) that can trap and kill invading microorganisms in vitro and in vivo. To determine if a similar mechanism is present in chicken heterophils, hydrogen peroxide (H2O2) and phorbol myristate acetate (PMA) were used for stimulation of blood-derived heterophils. Stimulated heterophils produced structures that were characterized using immunocytochemistry and confocal microscopy as heterophil extracellular traps (HETs). Released HETs contained DNA, histone–DNA complex and elastase from heterophil cytoplasmic granules. HETs released from chicken heterophils are structurally similar to NETs found in mammalian and fish neutrophils. Extracellular DNA released from heterophils was quantified by Picogreen assay. Stimulation with PMA or H2O2 significantly increased the HET-DNA release index in vitro compared to non-stimulated heterophils (1.11 ± 0.04 and 1.55 ± 0.10, respectively), and H2O2 stimulation induced significantly higher HET-DNA release than PMA (P < 0.001). Thus, HETs are now characterized as an important heterophil-mediated defense mechanism in chickens.
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