Dual-Luciferase TM Reporter Assay: An Advanced Co-Reporter Technology Integrating Firefly and Renilla Luciferase Assays

In the quantitation of gene expression using firefly luciferase, a second reporter gene is commonly used to minimize experimental variability. However, traditional co-reporters (e.g., CAT, beta-Gal or GUS) are inconvenient due to differences in their respective assay chemistries, handling requirements and measurement characteristics. Promega introduces a superior dual-reporter technology integrating the assay of firefly luciferase with the Renilla luciferase assay. The Dual-LuciferaseTM Reporter Assay System*, in combination with the pRL Vectors which express Renilla luciferase as the second reporter, offers the exceptional speed, sensitivity and convenience of two luciferase reporter assays in a single-tube format. The system also includes Passive Lysis Buffer, formulated to provide quantitative solubilization of both luciferases from mammalian cells cultured in multi-well plates without the need for individual processing of each sample. The Dual-LuciferaseTM Reporter Assay System will provide immediate benefit to researchers currently using any experimental firefly luciferase reporter vector.