Cellulose Synthase-Like CslF Genes Mediate the Synthesis of Cell Wall (1,3;1,4)-ß- d -Glucans

A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-β- d -glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-β- d -glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis , we detected (1,3;1,4)-β- d -glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-β- d -glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-β- d -glucan biosynthesis.