酶
计算生物学
药物发现
生物
生物化学
仿形(计算机编程)
蛋白质组学
化学生物学
化学
计算机科学
基因
操作系统
作者
Micah J. Niphakis,Benjamin F. Cravatt
标识
DOI:10.1146/annurev-biochem-060713-035708
摘要
Eukaryotic and prokaryotic organisms possess huge numbers of uncharacterized enzymes. Selective inhibitors offer powerful probes for assigning functions to enzymes in native biological systems. Here, we discuss how the chemical proteomic platform activity-based protein profiling (ABPP) can be implemented to discover selective and in vivo–active inhibitors for enzymes. We further describe how these inhibitors have been used to delineate the biochemical and cellular functions of enzymes, leading to the discovery of metabolic and signaling pathways that make important contributions to human physiology and disease. These studies demonstrate the value of selective chemical probes as drivers of biological inquiry.
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