A Fruiting Body Tissue Method for Efficient Agrobacterium -Mediated Transformation of Agaricus bisporus

ABSTRACT We describe a modified Agrobacterium -mediated method for the efficient transformation of Agaricus bisporus . Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.