Objective to study the hypnotic effects of the Yining.Methods Its effects to the autonomic activities of mice and the hymnotid duration of pentobarbital and the hypnotic effect of a subthreshold dose of pentobarbital were observed.Results Yining distinctly decresed the autonomic activity and incresed the hypnotic effect of a hypnotic subthreshold of pentobarbital and prolonged the duration of sleeping of pentobarbital mice. All effects were dosage dependent.Conclusion Yining has the effects of hypnotis.