The study analyses effect of different fertilization treatments on there kinds combined formation of humus in the long-term vegetable fertilization experimental field. The main results are shown as follows: the treatments of long-term fertilization of organic manure and organic manure combined with chemical fertilizers, content of combined loose-combined humus , tight-combined humus,steady-combined humus and the ratio of loose-combined humus and tight-combined humus are increase with the years growth, but the treatments of long-term non fertilization and long-term fertilization of organic manure ,only the content of tight-combined humus are increase, the content of loose-combined humus and steady-combined humus as well as the ratio of loose-combined humus and tight-combined humus are decrease with the years growth.