Expanding dendritic cells derived from mouse bone-marrow in vitro

OBJECTIVE:To establish a method of expanding dendritic cells from mouse bone marrow in vitro.METHODS: Mononuclear cells isolated from mouse bone marrow were induced into dendritic cells by being cultured in GM-CSF and IL-4,and then examined from their morphology,phenotype and function.RESULTS: A large number of morphologically typical dendritic cells were observed after the culturing for 8 days,and high expressed CD11c(83.19%),MHC-Ⅱ(95.25%),and CD86(89.24%),and their capacity to stimulate primary T cell responses in the mixed leukocyte reaction(MLR) was shown strongly.CONCLUSION: A large number of dendritic cells can be generated by culturing the mononuclear cells derived from mouse bone marrow in vitro,establishing a foundation for producing the anti-tumour vaccine in next step.