In order to investigate the effect of different substrates on extracellular enzyme activities,three substrate formulas were designed to determine the extracellular enzyme activities of carboxmethycellulase,hemicellulase,laccase and amylase.The thick liquid enzymes at six developmental stages were drawed.The result indicated that the extracellular enzyme activities exhibited different levels in different substrates and different developmental stages.But change trends at the levels of each individual enzyme observed throughout the sampling period were generally very similar irrespective of the composition of the cultivation substrate.Different substrates have much effect on extracellular enzyme activities,but less to changing trend.