Study on Extraction of Chlorogenic Acid in Flos Lonicerae

Objective To select the optimal extracting technique of Honeysuckle. Method Orthogonal design and HPLC were used to determine the total of chlorogenic acid contents as the index under different influences. Results The optimal extracting conditions were adding 75% alcohol and refluxing extract for 3 times with 0.5 hour each time, and the alcohol used for these three times extraction was 10 times、8 times、6 times respectively. Conclusion The methods has high transferring rate of chlorogenic acid and can increase the utilization rate of Honeysuckle.